The Indigo Method
With the indigo method, indigo trisulfonate dye immediately reacts with ozone. The color of the blue dye decreases in intensity in proportion to the amount of ozone present in the sample. The test reagent is formulated with malonic acid to prevent interference from up to at least 10 ppm chlorine. Results are expressed as ppm (mg/L) O3.
The CHEMetrics Indigo Ozone Vacu-vials® Refill Kit employs an innovative “self-zeroing” feature to eliminate the need to generate a reagent blank. Each Vacu-vials® ampoule is measured before and after being snapped in sample. The change in color intensity, measured in absorbance, between reagent in the unsnapped and snapped ampoule is used to determine the ozone concentration of the sample.
References: Bader H. and J. Hoigné, “Determination of Ozone in Water by the Indigo Method,” Water Research Vol. 15, pp. 449-456, 1981. APHA Standard Methods, 22nd ed., Method 4500-03 B (1997).